82P Correlations of androgen receptor (AR) and transforming growth factor beta receptor type 2 (TGFbR2) expression in prostate cancer (PCa)

Transforming growth factor beta (TGFb) is a potent inducer of epithelial-mesenchymal transition. Of all members the TGFb receptor family only TGFbR2 capable direct binding to ligand. In experimental conditions interactions were shown between and AR signaling pathways, often leading negative feedback loops. However, data on expression levels both receptors in clinical cancer samples are scarce. was studied 31 radical prostatectomy specimens. Double immunofluorescence used with primary mouse monoclonal anti-AR (BioGenex, 1:100 dilution) rabbit polyclonal anti-TGFbR2 (elabscience, 1:100) antibodies (Ab) secondary goat Ab (ThermoFischer, 1:200) labelled Alexa Fluor 488 555. 3-12 random high-power fields (HPF) (x400) per case (total 277 HPFs) studied, staining characteristics assessed semi-quantitatively (intensity: 1 – weak, 2 moderate, 3 strong; proportion stained cells: 0 absent, up 1/3, 1/3 2/3, more than 2/3). nuclear seen mostly PCa cells. Staining stroma weak present cells nuclei membranes, former being prevalent. The number HPFs various table.Table: 82PScoreAR, nuclear, cellsTGFbR2, cellsNuclearMembranousIntensityProportionIntensityProportionIntensityProportion01212373919719711797018311965572636646671510323129952013 Open table new tab reduced PCa. expressed using this Ab, while membranous 20 (64.5%) cases, but low single glands. Statistically significant correlation intensity same cell, usually weaker (p<0,05). No correlations found sample cohort presence expression, as well no markers stage or Gleason score. samples’ directly correlated that nuclei, despite loops proteins. biological mechanisms significance need further investigation.